The AOUFOUCHI team participate in this article : Tet2 deficiency promotes IgG1+ B-cell expansion and differentiation blockade through deregulation of the Nfkbia-c-Rel axis
G. Matos Rodrigues co-authors this work led by the Schatz lab describing the roles of ELOF1 during somatic hypermutation and class switch recombination.
G. Matos Rodrigues co-authors this work of the Sleckman lab on the roles of Senataxin and DNA-PKcs during DSB by NHEJ.
In this work, authored by G. Matos-Rodrigues. CRISPR-Cas9 nickases, were used to study how replication forks respond to single-strand DNA breaks.
Leading-strand nicks cause fork collapse, forming single-ended DSBs repaired via homologous recombination.
If unrepaired, neighboring forks can create double-ended DSBs, promoting genome instability in HR-deficient cells.
Lagging-strand nicks can also generate double-ended DSBs when bypassed by replication.
BRCA1 is not needed for resection here but counters 53BP1 to support RAD51 loading and fork stability.
B. Rondinelli co-authors this review covering the diveristy of Histone varints and their importance for Genome Integrity.
N. Petryk co-authors this review of the mechanisms of DNA methylation in mammals and its biological importance.
B. Rondinelli co-authors this article of the D’Andrea lab describing the roles of EZH2 at stalled replication forks.
Polo-like kinase 1 (PLK1) plays key roles during mitosis, prompting the development of PLK1 inhibitors for anticancer therapy. We recently determined that PLK1 is crucially required for entry into mitosis. Hence, we discuss the potential and limitations of PLK1 inhibition strategies to promote mitotic arrest and death of cancer cells.