The ATR/Chk1 pathway regulates cell cycle progression, especially during DNA damage by inducing arrest for repair. Beyond damage response, Chk1 prevents premature mitosis during normal S phase, maintaining genome integrity. The Gavet team developed a FRET-based reporter to monitor Chk1 activity in live single cells with high sensitivity. This tool reveals Chk1 dynamics during normal and stressed conditions.
« DNA adducting carcinogens exacerbate APOBEC mutagenesis and carcinogenesis »
Reuben Harris (UT San Antonio, US)
01/10/2025 at 11h
Salle Pierre Denoix (15th Floor Gustave Roussy main builiding)
The 7th German-French DNA repair meeting will be held this November 5th-7th in Paris. The unit is this time involved as hosting/organizing committee of the meeting. We will wellcome you soon!
GR wellcomes Lisa Mangiante and Elsa Bernard to give two talks for the selection of candidates to the « Chair Fondation Gustave » . The seminars will take place October 2nd 2025 at Salle Pierre Denoix (+15 floor in GR main builidng).
The Kannouche team participate in this article presenting the results of the phase 2 ICARUS-BREAST01 study. The study showed a 53.5% response rate to HER3-DXd (an antibody–drug conjugate) in HR+HER2− metastatic breast cancer after CDK4/6 inhibitor and chemotherapy treatment.
L’équipe des « Gardiens du Génome » cartonne au KOC du 13/09/2025
This is a collaborative effort of the Kannouche and Mazon/LeCam teams of the unit. DNA lesions during S phase challenge genome stability, managed by DNA damage tolerance (DDT) via TLS polymerases or recombination. Using electron microscopy, we show TLS polymerase η acts at replication forks to prevent gaps after UV damage. We outline a timeline of UV damage tolerance highlighting polη’s crucial role in DNA synthesis continuity.
La retraite de l’unité aura lieu le 16 Octobre 2025 à la cité U (Fondation Deutsch de la Meurthe). RDV à 8:45!
Fondation Deutsch de la Meurthe au CIUP (RER cité U).
Attention ! :
– L’Accès Piéton se fera par le 37 Boulevard Jourdan, 75014 PARIS
– L’Accès voiture se fera par les 27/29/31 Boulevard Jourdan 75014 PARIS
The Gavet team developed a FRET-based sensor to specifically monitor Chk1 activity during replication stress (RS).
Basal Chk1 activity during S phase depends on replication origin firing, with RS triggering stepwise Chk1 over-activation that delays S-phase.
Chk1 is inactivated upon replication completion but reactivates in some G2 cells to block premature mitosis.
Cells can override active Chk1 signaling to enter mitosis, revealing checkpoint adaptation.
Beatrice Rondinelli co-authors a study revealing that pediatric high-grade gliomas (pHGG) with H3.3 mutations exhibit aberrant DNA repair promoting genome instability. Their findings suggest new therapeutic targets for treating H3.3 mutant pHGG.